Fourth-generation sequencers are increasingly being used in the field of oncology research. Sequencing of circulating tumor DNA (ctDNA) enables non-invasive detection of tumor mutations, providing an effective means for early cancer screening. Gene panel analysis can quickly and accurately identify mutations in multiple tumor-associated genes, helping to develop individualized therapies. In addition, mRNA sequencing can reveal changes in gene expression in tumor cells, providing insight into understanding the mechanisms of tumor development.