In the fields of environmental and food research, fourth-generation sequencers play a key role. Through environmental testing, microorganisms, pollutants and other harmful substances in the environment can be monitored, providing scientific basis for environmental protection and pollution prevention. In terms of food safety, sequencing technology can track the sources of microbial contamination in food and ensure food quality and safety. In addition, research on genetically modified organisms (GMOs) and agriculture can reveal the characteristics of crop genomes through sequencing, providing sustainable solutions for agricultural production.