Axbio Publication Alert! | AxiLona AXP-100 Long-Read Sequencing Powers Gut Microbiome Research

July 28th, 2024 - In 2023, Axbio initiated a research collaboration with Professor Peter Belenky's group at Brown University (USA) to explore applications of nanopore-based gene sequencing technology in microbiome studies.

Following the successful deployment of the AxiLona AXP-100 sequencing platform, the research team continued testing its capabilities in microbial analysis. In July 2024, their findings were published in the journal Frontiers in Microbiomes, highlighting the advantages of long-read sequencing in gut microbiome research1.

The 16S rRNA gene is approximately 1.5 kb in length. Traditional second-generation sequencing platforms with 150 bp read lengths can only read relatively short parts of the whole 16S rRNA gene leading to incomplete taxonomic resolution. Based on data, generated in the publication, the research team concluded that high-accuracy long-read sequencing with the AXP-100 platform enables full-length 16S rRNA sequencing, providing higher-resolution bacterial community analysis. This advancement simplifies and enhances the reliability of gut microbiome analysis, particularly for complex samples such as intestinal microbiota.

The study reinforces the potential of long-read sequencing to improve microbiome research by offering more complete, high-confidence microbial profiling. 

Stay tuned for more breakthroughs powered by Axbio's cutting-edge sequencing technologies!

[1] Curtis-Joseph Noelle , Peterson Rachel , Brown Claire E. , Beekman Chapman , Belenky Peter (2024). Mouse diet and vendor impact microbiome perturbation and recovery from early-life pulses of amoxicillin. Frontiers in Microbiomes. DOI:10.3389/frmbi.2024.1432202